AI Article Synopsis
- The advancement of automated DNA and peptide synthesis has significantly improved access to defined biopolymers, enhancing biological research and medical practices.
- The article discusses how the Glyconeer 2.1 synthesizer, combined with monosaccharide building blocks and a polystyrene support, enables the quick assembly of oligosaccharides.
- Standardized purification and quality control methods ensure that these synthetic glycans are effective for use in glycan arrays, diagnostic tools, and carbohydrate-based vaccines.
Article Abstract
Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydrate-based vaccines.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5410834 | PMC |
| http://dx.doi.org/10.1073/pnas.1700141114 | DOI Listing |
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