Human PIWIL2, aka HILI, is a member of PIWI protein family and overexpresses in various tumors. However, the underlying mechanisms of HILI in tumorigenesis remain largely unknown. TBCB has a critical role in regulating microtubule dynamics and is overexpressed in many cancers. Here we report that HILI inhibits Gigaxonin-mediated TBCB ubiquitination and degradation by interacting with TBCB, promoting the binding between HSP90 and TBCB, and suppressing the interaction between Gigaxonin and TBCB. Meanwhile, HILI can also reduce phosphorylation level of TBCB induced by PAK1. Our results showed that HILI suppresses microtubule polymerization and promotes cell proliferation, migration and invasion via TBCB for the first time, revealing a novel mechanism for HILI in tumorigenesis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385498 | PMC |
| http://dx.doi.org/10.1038/srep46376 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Diagnostic value and safety of transbronchial cryobiopsy in postoperative complications after lung transplantation].
Zhonghua Jie He He Hu Xi Za Zhi
November 2024
Department of Lung Transplantation, Center of Respiratory Medicine, China-Japan Friendship Hospital, National Center for Respiratory Medicine, Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, National Clinical Research Center for Respiratory Diseases, WHO Collaborating Centre for Tobacco Cessation and Respiratory Diseases Prevention, Beijing 100029, China.
Article Synopsis
- A study compared the effectiveness and safety of transbronchial cryobiopsy (TBCB) versus transbronchial lung biopsy (TBLB) for diagnosing postoperative complications in lung transplant patients from June 2017 to June 2024.
- Results showed TBCB had significantly higher pathological (85.7% vs. 45.7%) and clinical diagnosis rates (94.2% vs. 69.9%) compared to TBLB, although both procedures had similar rates of severe complications like bleeding and pneumothorax.
- The most common complications identified after TBCB included chronic lung allograft dysfunction and antibody mediated rejection, whereas TBLB showed higher occurrence of antibody mediated rejection and
Lepidic Pulmonary Metastasis from Pancreatic Carcinoma Mimicking Organizing Pneumonia, Diagnosed by a Transbronchial Cryobiopsy.
Intern Med
September 2024
Department of Respiratory Medicine, NHO Okayama Medical Center, Japan.
Article Synopsis
- A 78-year-old man with pancreatic cancer had a chest CT that showed a growing area in his right lung, leading doctors to suspect organizing pneumonia after initial biopsies showed no cancer.
- Despite these findings, his levels of a tumor marker (carbohydrate antigen 19-9) were rising monthly, raising fears of possible lung cancer metastasis.
- A transbronchial cryobiopsy (TBCB) was conducted to confirm pulmonary metastasis, highlighting the importance of differentiating between lung consolidation due to cancer and organizing pneumonia.
[Differential diagnosis of coccidioidomycosis manifested by peripheral pulmonary lesion].
Khirurgiia (Mosk)
August 2024
Central TB Research Institute (CTRI), Moscow, Russia.
We present this clinical case as a demonstration of difficulties in differential diagnosis of pulmonary coccidioidomycosis. Differential diagnostics of peripheral pulmonary lesion performed using bronchoscopy with BAL and TBCB and video-assisted thoracic surgery (VATS) biopsy. Diagnostic specimens were tested using microbiological (luminescent microscopy, culture (BACTEC MGIT960 and Lowenstein-Jensen Medium), RT-PCR, cytological and morphological (hematoxylin-eosin, Ziehl-Neelsen, PAS, Grocott methenamine silver (GMS) stainings) examinations.
View Article and Find Full Text PDFThe application of bronchoscopy in the assessment of immune checkpoint inhibitor-related pneumonitis severity and recurrence.
Sci Rep
July 2024
Department of Respiratory and Critical Care Medicine, Peking University First Hospital, NO.8 Xishiku Street, Xicheng District, Beijing, 100034, China.
To explore the role of bronchoscopy for the assessment of checkpoint inhibitor pneumonitis (CIP), a retrospective single-center study was conducted to assess patients diagnosed with CIP at grade 2 or above and also underwent bronchoscopy between January 2020 and December 2022. Clinical data and bronchoscopic findings were recorded. The treatment data and prognosis information were collected.
View Article and Find Full Text PDFA novel technique for conducting flexible bronchoscopy cryobiopsy under conscious sedation: An observational study.
Medicine (Baltimore)
July 2024
Department of Medical Surgical and Health Sciences, Pulmonology Unit, University Hospital of Cattinara, University of Trieste, Trieste, Italy.
Transbronchial lung cryobiopsy (TBCB) is a reliable method for obtaining histopathological findings in interstitial lung diseases. TBCB is traditionally performed during rigid bronchoscopy, positioning an endobronchial balloon blocker to facilitate bleeding management. Therefore, it can be challenging to implement in Centers without access to anesthesiologic support or dedicated beds for endoscopic procedures.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!