AI Article Synopsis
- This study focuses on identifying and characterizing six common gene cassettes from bacterial integrons using quick and easy LAMP (loop-mediated isothermal amplification) assays.
- The gene cassettes analyzed include dfrA12, dfrA17, aadA2, aadA5, orfF, and bla, with specific attention to optimizing the LAMP assays for effective results.
- Ultimately, the approach demonstrated a perfect detection rate, finding 272 isolates with the gene cassettes and showing no false positives in 685 integron-negative bacteria tested.
Article Abstract
In this study, a number of frequently detected gene cassettes from bacterial integrons have been detected and characterized by rapid and simple loop-mediated isothermal amplification (LAMP) assays. Six gene cassettes commonly found in class 1 integrons were studied, including dfrA12, dfrA17, aadA2, aadA5, orfF, and bla. Primers design, sensitivity, specificity, optimization of each LAMP assay, as well as application of the developed 6 individual LAMP assays on a large scale of bacteria, had been conducted. The optimal amplification was obtained with temperature as 65 °C, reaction time span as 45 min and volume as 25 μl. For application, 272 isolates with various gene cassettes yielded expectable positive amplicons and other 685 integron-negative bacteria showed negative results for the LAMP assays, totaling 100% detection rate and specificity.
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| http://dx.doi.org/10.1016/j.micpath.2017.04.006 | DOI Listing |
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