A novel mitochondria-targeted near-infrared fluorescence probe for ultrafast and ratiometric detection of SO derivatives in live cells.

A novel mitochondria-targeted ratiometric near-infrared fluorescence probe NDMBT for Sulfur dioxide (SO) derivatives was constructed based on the SO derivatives-triggered Michael addition reaction. It displayed ultrafast response time (within 10s), large hypsochromic shift (260nm), high photostability, excellent selectivity and high sensitivity in aqueous media with a detection limit of 43nM. More importantly, it was successfully applied to imaging of the enzymatically generated SO derivatives in mitochondria of live cells.