AI Article Synopsis
- Most bacteria swim using a flagellum, which has a basal body critical for its motor function.
- Cryo-electron tomography studies have shown that the flagellar basal body features core structures like rods and various rings (C, M, S, L, P) and may also have extra rings in some bacteria.
- The text outlines a protocol for isolating the flagellar basal body from the marine bacterium Vibrio alginolyticus to study additional structures called T ring and H ring.
Article Abstract
Most bacteria can swim by rotating the flagellum. The basal body of the flagellum is an essential part for this motor function. Recent comprehensive analysis of the flagellar basal body structures across bacteria by cryo-electron tomography has revealed that they all share core structures, the rod, and rings: the C ring, M ring, S ring, L ring, and P ring. Furthermore, it also has uncovered that in some bacteria, there are extra ring structures in the periplasmic space and outer-membrane. Here, we describe a protocol to isolate the basal body of the flagellar basal body from a marine bacterium, Vibrio alginolyticus, for structural analysis of additional ring structures, the T ring and H ring.
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| http://dx.doi.org/10.1007/978-1-4939-6927-2_20 | DOI Listing |
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