In situ synthesis of silver nanoparticles into TEMPO-mediated oxidized bacterial cellulose and their antivibriocidal activity against shrimp pathogens.

Vibrionaceae is bifolded drug resistant emerging pathogens, active in various aquaculture sectors especially in shrimp culture around the world. V. parahaemolyticus are most dreadful viral outbreaks to shrimp culture. During the last decade the shrimp production level has been increased and also accompanied with various diseases. In the present research bacterial cellulose (BC) membrane produced by Gluconacetobacter xylinus in cell suspension. Further, TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical) oxidation was done to activate carboxyl group subsequently, TEMPO oxidized BC immersed in AgNO solution to generate AgNP anchored with BC to increase vibriocidal activity. Investigation of pure BC, TEMPO-BC and AgNP deposited BC was done by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and X-ray diffraction (XRD). Further vibriocidal effect was observed against the pathogen V. parahaemolyticus and V. harveyi. We found AgNP-BC more effective as compare pure BC, TEMP-BC, and AgNO Therefore AgNP deposited BC, is promising alternative to control the shrimp pathogen.