MicroRNA-379 inhibits the proliferation, migration and invasion of human osteosarcoma cells by targetting EIF4G2.

Osteosarcoma (OS) is an aggressive malignant mesenchymal neoplasm amongst adolescents. The aim of the present study was to explore the various modes of action that has on the proliferation, migration, and invasion of human OS cells. achieves this by targetting eukaryotic initiation factor 4GII (EIF4G2). Human OS cell lines U2OS and MG-63 were selected and assigned into blank, mimics, mimic negative control (NC), inhibitors, inhibitor NC, EIF4G2 shRNA, control shRNA, and inhibitor + EIF4G2 shRNA group. The expression and mRNA expression were detected utilising quantitative real-time PCR (qRT-PCR) and the EIF4G2 protein expression using Western blotting. MTT assay, scratch test, Transwell assay, and flow cytometry were performed to determine the proliferation, migration, invasion, and cell cycle, respectively. In comparison with the mimic NC group, the mimics group had decreased EIF4G2 expression; the inhibitors group indicated an increased EIF4G2 expression. Compared with the control shRNA group, the EIF4G2 expression was lower in the EIF4G2 shRNA group and the expression was dropped in the inhibitor + EIF4G2 shRNA group. The proliferation, migration, and invasion abilities of OS cells were reduced in the mimics and EIF4G2 shRNA groups. The percentage of OS cells at the G/G stage was increased, and the percentage at the S-stage was decreased in the mimics and EIF4G2 shRNA groups. may inhibit the proliferation, migration and invasion of OS cells through the down-regulation of EIF4G2.