AI Article Synopsis
- Aneuploidy, a common feature in human tumors, lacks thorough understanding in terms of how it affects cell function and behavior.
- Researchers used flow cytometry to examine around 300 cell surface proteins in various aneuploid models, discovering new biomarkers like CD230/PrP and CD73 that are overexpressed in these cells.
- The study found that these biomarkers are linked to increased cellular stress, with more CD73-positive cells in dense cultures and heightened CD230/PrP levels in serum-deprived conditions, revealing clues about the biology of aneuploid cells.
Article Abstract
Aneuploidy is a hallmark of most human tumors, but the molecular physiology of aneuploid cells is not well characterized. In this study, we screened cell surface biomarkers of approximately 300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as cell lines, patient samples, and mouse models. Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrP and the immunosuppressive cell surface enzyme ecto-5'-nucleotidase CD73. Functional analyses associated these alterations with increased cellular stress. An increased number of CD73 cells was observed in confluent cultures in aneuploid cells relative to their diploid counterparts. An elevated expression in CD230/PrP was observed in serum-deprived cells in association with increased generation of reactive oxygen species. Overall, our work identified biomarkers of aneuploid karyotypes, which suggest insights into the underlying molecular physiology of aneuploid cells. .
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| http://dx.doi.org/10.1158/0008-5472.CAN-16-3052 | DOI Listing |
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