Background: In the vitrification of embryos, dimethyl sulfoxide (DMSO) is one of the most effective cryoprotectant agents (CPAs), but cytotoxic effects of DMSO on embryos are well known. Carboxylated poly-L-lysine (CPLL) has been identified as an effective cryoprotectant of cultured cell lines and mammalian oocytes.
Objective: To evaluate the efficacy and safety of CPLL as a CPA for developmental stage embryos.
Materials And Methods: Mouse 8-cell embryos and blastocysts were vitrified with ethylene glycol (EG), DMSO/EG, or CPLL/EG and the developmental potency assessed in vitro.
Results: In 8-cell embryos, there were no differences between the levels of survival and developmental progress into the blastocyst stage in each solution. At the blastocyst stage, the proportion of dead cells was significantly higher in the EG compared with other solutions. In contrast, there were no differences between the DMSO/EG and CPLL/EG.
Conclusion: These results indicate that CPLL can be used as a replacement for DMSO in the vitrification of mouse embryos.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Composite antibacterial hydrogels based on two natural products pullulan and ε-poly-l-lysine for burn wound healing.
Int J Biol Macromol
October 2024
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics (LICP), Chinese Academy of Sciences (CAS), Lanzhou 730000, China. Electronic address:
Antibacterial hydrogels as burn wound dressings are capable of efficaciously defending against bacterial infection and accelerating burn wound healing. Thus far, a large plethora of antibacterial hydrogels have adopted numerous components and intricate preparation processes, yet restricting their practical industrialization applications. Simple and effective preparation methods of antibacterial hydrogels are hence urgently needed.
View Article and Find Full Text PDFCompact Polyelectrolyte Complexes of Poly(l-Lysine) and Anionic Polysaccharides.
Biomacromolecules
August 2024
Department of Chemistry, The University of Western Ontario, 1151 Richmond St., London, Ontario N6A 5B7, Canada.
Compact polyelectrolyte complexes (CoPECs) can exhibit mechanical properties similar to those of biological tissues and other interesting properties, such as self-healing. To date, a variety of CoPECs prepared from synthetic polyelectrolytes have been investigated, but there are very few examples based entirely on biopolymers. We describe here an investigation of CoPECs based on poly(l-lysine) (PLL) with sodium hyaluronate (HA) and alginate (Alg).
View Article and Find Full Text PDFCarboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol.
Cells
November 2023
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, Japan.
Sperm DNA fragmentation (SDF) that occurs during the freezing-thawing of sperm may negatively impact the treatment outcomes of assisted reproductive technologies (ART). In a previous study, we developed a human sperm cryopreservation reagent containing carboxylated poly-L-lysine (CPLL) that reduced SDF after freeze-thawing compared with clinically popular cryopreservation reagents containing human serum albumin. However, it is unclear whether CPLL reduces SDF, as it differed from the constituents of the commercial cryopreservation reagents used for comparison.
View Article and Find Full Text PDFSensitive and rapid detection of bacterial endotoxin with a functional carbon nanotube field-effect transistor biosensor.
Talanta
January 2024
School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan, 430065, PR China. Electronic address:
Endotoxin in the blood can cause unexplained fever, and even death due to endotoxemia and bacteremia. Rapid and sensitive detection of endotoxin has become a priority event to intervene in the occurrence of dangerous diseases in time. In this context, a carbon nanotubes-based field-effect transistor (CNTs FET) nanosensor is developed to realize the rapid, label-free and sensitive detection of endotoxin.
View Article and Find Full Text PDFCryopreservation of engineered hair follicle germs for hair regenerative medicine.
J Biosci Bioeng
September 2023
Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa 240-8501, Japan; Kanagawa Institute of Industrial Science and Technology, 3-2-1 Sakado Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan. Electronic address:
Hair regenerative medicine must involve practical procedures, such as cryopreservation of tissue grafts. This can aid in evaluating tissue safety and quality, as well as transportation to a clinic and multiple transplants. Hair follicle germs (HFGs), identified during in vivo development, are considered effective tissue grafts for hair regenerative medicine.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!