A PCR-dipstick chromatography technique was designed and evaluated for differential identification of , , , and carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.
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| http://dx.doi.org/10.1128/AAC.00067-17 | DOI Listing |
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