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How to gather useful and valuable information from protein binding measurements using Langmuir lipid monolayers. | LitMetric

How to gather useful and valuable information from protein binding measurements using Langmuir lipid monolayers.

Adv Colloid Interface Sci

CUO-Recherche, Hôpital du Saint-Sacrement, Centre de recherche du CHU de Québec and Département d'ophtalmologie, Faculté de médecine, and Regroupement stratégique PROTEO, Université Laval, Québec, Québec, Canada. Electronic address:

Published: May 2017

AI Article Synopsis

Article Abstract

This review presents data on the influence of various experimental parameters on the binding of proteins onto Langmuir lipid monolayers. The users of the Langmuir methodology are often unaware of the importance of choosing appropriate experimental conditions to validate the data acquired with this method. The protein Retinitis pigmentosa 2 (RP2) has been used throughout this review to illustrate the influence of these experimental parameters on the data gathered with Langmuir monolayers. The methods detailed in this review include the determination of protein binding parameters from the measurement of adsorption isotherms, infrared spectra of the protein in solution and in monolayers, ellipsometric isotherms and fluorescence micrographs.

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Source
http://dx.doi.org/10.1016/j.cis.2017.03.004DOI Listing

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