was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the MxaY with the EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of and the fluorescence at 0.01% of methanol, respectively. These results suggested that was successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.
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