AI Article Synopsis
- Researchers are investigating new nucleosidic scaffolds for synthetic biology to create artificial genetic polymers with specific properties.
- A new bicyclo-DNA triphosphate (7',5'-bc-TTP) has been synthesized, showing promise for developing new xenonucleic acids (XNAs).
- While 7',5'-bc-TTP can be incorporated into DNA chains and supports XNA synthesis, its use is limited by misincorporation issues with dATP due to its slower incorporation rate.
Article Abstract
The selection of artificial genetic polymers with tailor-made properties for their application in synthetic biology requires the exploration of new nucleosidic scaffolds that can be used in selection experiments. Herein, we describe the synthesis of a bicyclo-DNA triphosphate (i.e., 7',5'-bc-TTP) and show its potential to serve for the generation of new xenonucleic acids (XNAs) based on this scaffold. 7',5'-bc-TTP is a good substrate for Therminator DNA polymerase, and up to seven modified units can be incorporated into a growing DNA chain. In addition, this scaffold sustains XNA-dependent DNA synthesis and potentially also XNA-dependent XNA synthesis. However, DNA-dependent XNA synthesis on longer templates is hampered by competitive misincorporation of deoxyadenosine triphosphate (dATP) caused by the slow rate of incorporation of 7',5'-bc-TTP.
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| http://dx.doi.org/10.1002/asia.201700374 | DOI Listing |
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