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Nondestructive Mechanical Characterization of Bioengineered Tissues by Digital Holography.
ACS Biomater Sci Eng
January 2025
Mechanical Engineering Department, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, United States.
Mechanical properties of engineered connective tissues are critical for their success, yet modern sensors that measure physical qualities of tissues for quality control are invasive and destructive. The goal of this work was to develop a noncontact, nondestructive method to measure mechanical attributes of engineered skin substitutes during production without disturbing the sterile culture packaging. We optimized a digital holographic vibrometry (DHV) system to measure the mechanical behavior of Apligraf living cellular skin substitute through the clear packaging in multiple conditions: resting on solid agar as when the tissue is shipped, on liquid media in which it is grown, and freely suspended in air as occurs when the media is removed for feeding.
View Article and Find Full Text PDFVitamin C mesotherapy with microneedling for gingival depigmentation: A case study.
Clin Adv Periodontics
January 2025
Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India.
Background: Surgical methods of gingival depigmentation can be challenging, particularly if the gingival phenotype is thin due to the risk of gingival recession and bone exposure. Thus, exploring alternative, non-surgical, minimally invasive treatment modalities is warranted. In dermatology, vitamin C is extensively used for depigmentation and microneedling for collagen induction, with limited literature about its usage for improving gingival esthetics.
View Article and Find Full Text PDFTopical Application onto the Eyelid Skin: Is it a Feasible Delivery Route of Ophthalmic Drugs?
Mini Rev Med Chem
January 2025
Research Institute for Medicines (imed), Faculty of Pharmacy, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal.
Topical application of ophthalmic drugs remains to be the preferred delivery method. Eye drops lead the pharmaceutical forms due to ease of application. Despite the poor bioavailability of drugs administered topically, especially related to the dilution and excretion by tear fluid, the absence of controlled drug delivery, and the poor compliance within pediatric and senior populations, eye drops and ointments are still the first choices in eye--related disorders management.
View Article and Find Full Text PDFThe immunoglobulin of yolk and cerium oxide-based fibrous poly(L-lactide-co-glycolide)/gelatin dressings enable skin regeneration in an infectious wound model.
Mater Today Bio
February 2025
Department of Orthopedics, The Third Affiliated Hospital of Shandong First Medical University (Affiliated Hospital of Shandong Academy of Medical Sciences), NO.38, Wuyingshan Road, Tianqiao District, Jinan, 250031, China.
The bacterial infection and oxidative wound microenvironment delay skin repair and necessitate intelligent wound dressings to enable scarless wound healing. The immunoglobulin of yolk (IgY) exhibits immunotherapeutic potential for the potential treatment of antimicrobial-resistant pathogens, while cerium oxide nanoparticles (CeO NPs) could scavenge superoxide dismutase (SOD) and inflammation. The overarching objective of this study was to incorporate IgY and CeO NPs into poly(L-lactide-co-glycolide)/gelatin (PLGA/Gel)-based dressings (P/G@IYCe) for infected skin repair.
View Article and Find Full Text PDFExosome-carried miR-1248 from adipose-derived stem cells improves angiogenesis in diabetes-associated wounds.
Int J Biol Macromol
January 2025
Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, PR China; The 2011 Collaborative Innovation Center of Tissue Damage Repair and Regeneration Medicine, Affiliated Hospital of Zunyi Medical University, PR China; The Collaborative Innovation Center of Tissue Damage Repair and Regeneration Medicine, Zunyi Medical University, PR China. Electronic address:
Chronic non-healing wounds are a common complication of diabetes, marked by impaired angiogenesis. This study explores how exosomes (Exo-miR-1248) from miR-1248-overexpressing adipose-derived stem cells enhance diabetic wound healing by modulating endothelial cell function. Adipose-derived stem cells were transfected with a lentivirus carrying miR-1248 to produce Exo-miR-1248, isolated via differential centrifugation.
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