AI Article Synopsis
- The study aimed to explore the antifungal properties of Xylaria sp. BCC 1067 extract against the yeast Saccharomyces cerevisiae.
- Results indicated that the extract was effective, with specific concentrations displaying strong antifungal activity and a significant synergistic effect when combined with ketoconazole.
- The extract caused reactive oxygen species production and cell damage, but certain cellular components helped S. cerevisiae withstand its toxic effects, highlighting the extract's potential as a source of new antifungals.
Article Abstract
Aim: To investigate antifungal potential of Xylaria sp. BIOTEC culture collection (BCC) 1067 extract against the model yeast Saccharomyces cerevisiae.
Materials & Methods: Antifungal property of extract, reactive oxygen species levels and cell survival were determined, using selected deletion strains.
Results: Extract showed promising antifungal effect with minimal inhibitory concentration and minimal fungicidal concentration of 500 and 1000 mg/l, respectively. Strong synergy was observed with fractional inhibitory concentration index value of 0.185 for the combination of 60.0 and 0.5 mg/l of extract and ketoconazole, respectively. Extract-induced intracellular reactive oxygen species levels in some oxidant-prone strains and mediated plasma membrane rupture. Antioxidant regulator Yap1, efflux transporter Pdr5 and ascorbate were pivotal to protect S. cerevisiae from extract cytotoxicity.
Conclusion: Xylaria sp. BCC 1067 extract is a potentially valuable source of novel antifungals.
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| http://dx.doi.org/10.2217/fmb-2016-0151 | DOI Listing |
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