A flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic Lactobacillus reuteri.

J Microbiol Methods

Inflammation Immunobiology Section, Laboratory of Allergic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address:

Published: June 2017

Eosinophils are resident leukocytes of gut mucosa. Here we present a combined flow cytometric-antibiotic protection assay to identify mouse eosinophils capable of bacterial uptake, specifically, Gram-positive Lactobacillus reuteri, in studies performed ex vivo. The assay may be adapted for use in vivo.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443408PMC
http://dx.doi.org/10.1016/j.mimet.2017.03.015DOI Listing

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