AI Article Synopsis
- Antibody derivatives like Fabs and scFvs are being used to visualize difficult-to-detect protein subpopulations, enabling researchers to study processes like translation and protein modifications.
- This approach allows for precise imaging and quantification of translation initiation and elongation, as well as tracking post-translational modifications in real time.
- The review highlights the benefits and limitations of using antibody-based probes in live-cell imaging, suggesting that improvements in these probes could advance research into gene regulation.
Article Abstract
Antibody derivatives, such as antibody fragments (Fabs) and single-chain variable fragments (scFvs), are now being used to image traditionally hard-to-see protein subpopulations, including nascent polypeptides being translated and post-translationally modified proteins. This has allowed researchers to directly image and quantify, for the first time, translation initiation and elongation kinetics with single-transcript resolution and the temporal ordering and kinetics of post-translational histone and RNA polymerase II modifications. Here, we review these developments and discuss the strengths and weaknesses of live-cell imaging with antibody-based probes. Further development of these probes will increase their versatility and open new avenues of research for dissecting complex gene regulatory dynamics.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526335 | PMC |
| http://dx.doi.org/10.1016/j.tig.2017.02.003 | DOI Listing |
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