Background: We have previously reported a procedure for isolating and culturing biliary epithelial cells (BECs). The aim of this study was to reconsider the method for obtaining pure BECs using the mouse gallbladder.
Materials And Methods: Cells that were obtained from the gallbladder alone were sorted by fluorescence-activated cell sorting (FACS) for purifying based on the expression of the epithelial cell adhesion molecule (EpCAM). The viability rate was measured based on the negative expression of 7-aminoactinomycin D (7-AAD).
Results: More than 75% of cells from the gallbladder were determined to be pure BECs. An analysis of the EpCAM revealed that 73.3% of the cells were 7-AAD-negative. Finally, the 0.82×10 pure BECs that survived were obtained and seeded on a collagen gel plate. However, these pure BECs showed almost no proliferation.
Conclusion: Pure BECs could be accumulated using FACS. However, the number of BECs was insufficient for the culturing process.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411741 | PMC |
| http://dx.doi.org/10.21873/invivo.11041 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Instabilities of a Bose-Einstein condensate with mixed nonlinear and linear lattices.
Phys Rev E
April 2023
Department of Physics, Shanghai University, Shanghai 200444, China.
Bose-Einstein condensates (BECs) in periodic potentials generate interesting physics on the instabilities of Bloch states. The lowest-energy Bloch states of BECs in pure nonlinear lattices are dynamically and Landau unstable, which breaks down BEC superfluidity. In this paper we propose to use an out-of-phase linear lattice to stabilize them.
View Article and Find Full Text PDFOptimized protocol for translatome analysis of mouse brain endothelial cells.
PLoS One
September 2022
Neurovascular Unit Research Group, Korea Brain Research Institute, Daegu, South Korea.
Article Synopsis
- Brain endothelial cells (BECs) play a crucial role in brain health by delivering nutrients, removing waste, and protecting against toxins, with their impairment linked to diseases like Alzheimer's and Parkinson's.
- Current methods to analyze BECs often rely on mRNA levels, which don't always reflect actual protein production, but using RiboTag mice allows for better insights through translatome analysis.
- The study developed a new protocol to isolate pure mRNAs from BECs, which improves specificity and quality for molecular studies, making it a valuable tool for future BEC research.
Papain-based Single Cell Isolation of Primary Murine Brain Endothelial Cells Using Flow Cytometry.
Bio Protoc
November 2018
Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA.
Brain endothelial cells (BECs) form the integral component of the blood-brain barrier (BBB) which separates the systemic milieu from the brain parenchyma and protects the brain from pathogens and circulating factors. In order to study BEC biology, it was of particular interest to establish a method that enables researchers to investigate and understand the underlying molecular mechanisms regulating their function during homeostasis, aging and disease. Furthermore, due to the heterogeneity of the cerebrovasculature and different vessel types that comprise the BBB, it is of particular interest to isolate primary BECs for single cell analysis from various subregions of the brain, such as the neurogenic and highly vascularized hippocampus and to enrich for specific vessel types.
View Article and Find Full Text PDFA Modified Method for Purifying Gallbladder Epithelial Cells Using Fluorescence-activated Cell Sorting.
In Vivo
June 2017
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Background: We have previously reported a procedure for isolating and culturing biliary epithelial cells (BECs). The aim of this study was to reconsider the method for obtaining pure BECs using the mouse gallbladder.
Materials And Methods: Cells that were obtained from the gallbladder alone were sorted by fluorescence-activated cell sorting (FACS) for purifying based on the expression of the epithelial cell adhesion molecule (EpCAM).
Selective distillation phenomenon in two-species Bose-Einstein condensates in open boundary optical lattices.
Sci Rep
November 2015
Department of Physics, Applied Optics Beijing Area Major Laboratory, Beijing Normal University, Beijing 100875, China.
We investigate the formation of discrete breathers (DBs) and the dynamics of the mixture of two-species Bose-Einstein condensates (BECs) in open boundary optical lattices using the discrete nonlinear Schrödinger equations. The results show that the coupling of intra- and interspecies interaction can lead to the existence of pure single-species DBs and symbiotic DBs (i.e.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!