Twenty-one species of sponges were recorded under the class of Demospongiae and Calcareous sponges of which 19 species were new to Agatti reef. A total of 113 Sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. Five bacterial strains of sponge endosymbiotic bacteria (SEB) namely SEB32, SEB33, SEB36, SEB43 and SEB51 showed antimicrobial activity against virulent marine fish pathogens such as , , , , sp., sp., and . The secondary metabolites produced by SEB32 from sponge (Montagu, 1818) [48] was selected with broad range of antibacterial activity and subjected for production, characterization by series of chromatography techniques and spectroscopic methods. Based on the results of FT-IR and mass spectrometry, the active molecule was tentatively predicted as "Pyrrol" and the structure is Pyrrolo[1,2]pyrazine-1,4-dione, hexahydro- with molecular formula of C7H10N2O2. The LC of active molecule was 31 μg/ml and molecular weight of the metabolites was 154. The potential strain SEB32 was identified by gene sequence (GenBank Accession number JX985748) and identified as sp. from GenBank database.
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http://dx.doi.org/10.1016/j.btre.2016.06.001 | DOI Listing |
BMC Biotechnol
November 2024
College of Fisheries and Aquaculture Technology, Arab Academy for Science, Technology and Maritime Transport, Abu Qir, Alexandria, Egypt.
Pathogenic fungi employ numerous strategies to colonize plants, infect them, reduce crop yield and quality, and cause significant losses in agricultural production. The increasing use of chemical pesticides has led to various ecological and environmental issues, including the emergence of resistant weeds, soil compaction, and water pollution, all negatively impacting agricultural sustainability. Additionally, the extensive development of synthetic fungicides has adverse effects on animal and human health, prompting the exploration of alternative approaches and green strategies for phytopathogen control.
View Article and Find Full Text PDFProc Biol Sci
June 2023
School of Biological Sciences, Victoria University of Wellington, Wellington 6140, New Zealand.
Marine heatwaves are increasingly subjecting organisms to unprecedented stressful conditions, but the biological consequences of these events are still poorly understood. Here we experimentally tested the presence of carryover effects of heatwave conditions on the larval microbiome, settlers growth rate and metamorphosis duration of the temperate sponge . The microbial community of adult sponges changed significantly after ten days at 21°C.
View Article and Find Full Text PDFMicrobiol Spectr
June 2022
Center for Marine Drugs, State Key Laboratory of Oncogene and Related Genes, Department of Pharmacy, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Sponge microbiomes are typically profiled by analyzing the community DNA of whole tissues, which does not distinguish the taxa residing within sponge cells from extracellular microbes. To uncover the endosymbiotic microbiome, we separated the sponge cells to enrich the intracellular microbes. The intracellular bacterial community of sponge was initially assessed by amplicon sequencing, which indicated that it hosts three unique phyla not found in the extracellular and bulk tissue microbiomes.
View Article and Find Full Text PDFJ Eukaryot Microbiol
May 2021
Penn State University, University Park, Pennsylvania, 16802, USA.
The dinoflagellate family Symbiodiniaceae comprises numerous divergent genera containing species whose ecologies range from endosymbiotic to free-living. While many associate with invertebrates including corals, sea anemones, jellyfish, giant clams, and flatworms, others occur within the cytoplasm of large protists, most notably benthic foraminifera in the sub-family Soritinae. Recent systematic revisions to the Symbiodiniaceae left out formal naming of some divergent lineages because each lacked a representative type species to erect new genus names.
View Article and Find Full Text PDFAnim Microbiome
March 2021
BioSciences at Rice, Rice University, 6100 Main St, MS-140, Houston, TX, 77005, USA.
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