multi-antigen sequence typing for .

Microb Genom

1​Doherty Applied Microbial Genomics, Department of Microbiology & Immunology, University of Melbourne, at the Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.

Published: August 2016

Whole-genome sequencing (WGS) provides the highest resolution analysis for comparison of bacterial isolates in public health microbiology. However, although increasingly being used routinely for some pathogens such as and , the use of WGS is still limited for other organisms, such as . Multi-antigen sequence typing (NG-MAST) is the most widely performed typing method for epidemiological surveillance of gonorrhoea. Here, we present a command-line software tool for performing NG-MAST on assembled genome data. rapidly and accurately determined the NG-MAST of 630 assembled genomes, facilitating comparisons between WGS and previously published gonorrhoea epidemiological studies. The source code and user documentation are available at https://github.com/MDU-PHL/ngmaster.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320595PMC
http://dx.doi.org/10.1099/mgen.0.000076DOI Listing

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