Toluene is a pollutant catabolised through the interconnected pWW0 (TOL) and ortho-cleavage pathways of Pseudomonas putida mt-2, while upon succinate and toluene mixtures introduction in batch cultures grown on M9 medium, succinate was previously reported as non-repressing. The effect of a 40 times lower succinate concentration, as compared to literature values, was explored through systematic real-time qPCR monitoring of transcriptional kinetics of the key TOL Pu, Pm and ortho-cleavage PbenR, PbenA promoters in mixed-substrate experiments. Even succinate trace inhibited transcription leading to bi-modal promoters expression. Potential carbon catabolite repression mechanisms and novel expression patterns of promoters were unfolded. Lag phase was shortened and biomass growth levels increased compared to sole toluene biodegradation suggesting enhanced pollutant removal efficiency. The study stressed the noticeable effect of a preferred compound's left-over on the main route of a bioprocess, revealing the beneficiary supply of low preferred substrates concentrations to design optimal bioremediation strategies.
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http://dx.doi.org/10.1016/j.biortech.2017.03.082 | DOI Listing |
Itaconate is an immunomodulatory metabolite that alters mitochondrial metabolism and immune cell function. This organic acid is endogenously synthesized via tricarboxylic acid (TCA) metabolism downstream of TLR signaling. Itaconate-based treatment strategies are being explored to mitigate numerous inflammatory conditions.
View Article and Find Full Text PDFChemistry
December 2024
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, P. R. China.
Achieving red organic room temperature phosphorescence (RTP) remains a significant challenge, especially in a non-aromatic system. Herein, red RTP with emission at 605 and 645 nm is achieved through inducing and confining carboxyl dimer association (CDA), a unique hydrogen-bonded coupling red phosphorescence unit, in non-aromatic carbonized polymer dots (CPDs). The CPDs are synthesized via microwave method by using polyacrylic acid (PAA), succinic acid (SA), and traces of phosphoric acid as precursors.
View Article and Find Full Text PDFElife
December 2024
Department of Epigenetics, Van Andel Research Institute, Grand Rapids, United States.
Eur J Med Chem
January 2025
School of Pharmacy, Fudan University, Shanghai, 201203, PR China. Electronic address:
A series of integrin αβ targeting BODIPY-RGD conjugate were designed and synthesized. Their in vitro and in vivo fluorescence imaging behaviors were investigated. The small molecule compound was designed as an optical imaging near-infrared fluorescent dye which was combined RGD peptide with the meso-amide BODIPYs using succinic moiety as a spacer.
View Article and Find Full Text PDFSci Total Environ
December 2024
Department of Chemistry "Giacomo Ciamician", University of Bologna, Via S. Alberto 163, 48123 Ravenna, Italy; Interdepartmental Centre for Research in Environmental Sciences (CIRSA), Via S. Alberto 163, 48123 Ravenna, Italy. Electronic address:
The potential release in the environment and biological effects of chemicals like additives and non-intentionally added substances present in conventional plastics and bioplastics is an issue that could occur if these materials are not properly disposed of. Herein, seven leachates of biobased and biodegradable plastics made of polylactic acid (PLA), polybutylene succinate (PBS)/PLA blends, and starch-based blends (SB) were characterized and compared for the inorganic and organic additives present in the source materials. The main inorganic elements found in the leachates were Na, Mg, K, and Ca (0.
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