AI Article Synopsis
- The isolation of human cell types is difficult, leading researchers to explore alternative methods like direct conversion of human pluripotent stem cells (hPSCs) using transcription factors.
- By improving the efficiency of gene expression in hPSCs through a dual safe harbor gene-targeting strategy, researchers have developed a more effective way to generate various human cell types.
- The study showcases successful reprogramming of hPSCs into neurons, functional skeletal myocytes, and offers a fast method for producing human oligodendrocytes.
Article Abstract
The isolation or in vitro derivation of many human cell types remains challenging and inefficient. Direct conversion of human pluripotent stem cells (hPSCs) by forced expression of transcription factors provides a potential alternative. However, deficient inducible gene expression in hPSCs has compromised efficiencies of forward programming approaches. We have systematically optimized inducible gene expression in hPSCs using a dual genomic safe harbor gene-targeting strategy. This approach provides a powerful platform for the generation of human cell types by forward programming. We report robust and deterministic reprogramming of hPSCs into neurons and functional skeletal myocytes. Finally, we present a forward programming strategy for rapid and highly efficient generation of human oligodendrocytes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390118 | PMC |
| http://dx.doi.org/10.1016/j.stemcr.2017.02.016 | DOI Listing |
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