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Diversity and Multiple Infections of in Red Deer and Deer Keds.
Pathogens
December 2024
Department of Parasitology, Faculty of Science, Charles University, 128 00 Prague, Czech Republic.
Bartonellae are zoonotic pathogens with a broad range of reservoir hosts and vectors. To examine sylvatic reservoirs, tissue samples of red deer (, = 114) and their associated deer keds (, = 50; , = 272) collected in the Czech Republic were tested for the presence of using PCR at four loci (, , , ITS); PCR sensitivity was increased significantly by using primers modified for the detection of wildlife-associated bartonellae. One-third of the deer and 70% of the deer keds were positive; within the tested animal tissues, usually the spleen was positive.
View Article and Find Full Text PDFComprehensive phylogenetic analysis of newly detected rodent adenoviruses sheds light on ancient host-switches.
Mol Phylogenet Evol
January 2025
HUN-REN Veterinary Medical Research Institute, H-1143 Budapest, Hungary.
Here we provide a comprehensive update on the diversity and genetic relatedness of adenoviruses occurring in rodents. Extensive PCR screenings revealed the presence of adenoviral DNA in samples originating from representatives of 17 rodent species from four different suborders of Rodentia. Distinct sequences of 28 different adenoviruses were obtained from the positive samples.
View Article and Find Full Text PDFNext step towards point-of-care molecular diagnosis of female genital schistosomiasis (FGS): evaluation of an instrument-free LAMP procedure.
Front Parasitol
May 2024
Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands.
Detection of spp. DNA in gynaecological samples by quantitative real-time polymerase chain reaction (qPCR) is considered to be the reference diagnostic test for female genital schistosomiasis (FGS). However, qPCR needs expensive laboratory procedures and highly trained technicians.
View Article and Find Full Text PDF[Mini-barcode combined with ITS2 for identification of bulk Artemisiae Scopariae Herba].
Zhongguo Zhong Yao Za Zhi
December 2024
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
Artemisiae Scoporiae Herba is derived from Artemisia scoparia or A. capillaris. The accurate identification of the herbs, particularly when dealing with bulk samples, is critical for ensuring the quality and efficacy of the medicinal product.
View Article and Find Full Text PDFA Real-World Evidence Primer for Advanced Practice Providers: Integrating P-Reality X Into Shared Decision-Making for People With HR+/HER2- Metastatic Breast Cancer.
J Adv Pract Oncol
July 2024
UCSD Comprehensive Breast Health Center, La Jolla, California.
Advanced practice providers in oncology are now likely to encounter real-world data (RWD) studies in addition to data from randomized controlled trials (RCTs) in their practice. Real-world evidence derived from RWD can provide important information about a therapeutic agent's effectiveness outside of the confines of RCTs. It is important to understand how these studies are conducted and how data from these two types of studies can be interpreted and integrated for practical clinical use and shared decision-making.
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