AI Article Synopsis
- Arachidonic acid (AA) is biologically activated through various pathways, with 5-lipoxygenase (5-LO) being a key one.
- 5-Hydroxyeicosatetraenoic acid (5-HETE) is converted to 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), which is a strong attractant for immune cells like eosinophils and neutrophils.
- The research aims to understand the regulation of 5-oxo-ETE production and its implications for health by developing tools to purify the enzyme responsible for its synthesis, 5-hydroxyeicosanoid dehydrogenase (5-HED
Article Abstract
Arachidonic acid (AA) is converted to biologically active metabolites by different pathways, one of the most important of which is initiated by 5-lipoxygenase (5-LO). 5-Hydroxyeicosatetraenoic acid (5-HETE), although possessing only weak biological activity itself, is oxidized to 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), a potent chemoattractant for eosinophils and neutrophils. Our main goal is to determine how the biosynthesis of 5-oxo-ETE is regulated and to determine its pathophysiological roles. To achieve this task, we designed and synthesized affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase (5-HEDH), the enzyme responsible for the formation of 5-oxo-ETE.
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| http://dx.doi.org/10.1016/j.bmc.2016.10.017 | DOI Listing |
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