AI Article Synopsis
- A cDNA library was created from sweet potato leaf RNA, resulting in 789 cloned cDNA copies in E. coli and the design of 579 EST-SSR markers from these sequences.
- The EST-SSR markers, ranging from 100 to 499 bp in length, mainly consisted of dinucleotides and pentanucleotides, with several common motifs identified.
- Polymorphic analysis revealed 82 variable markers among 20 sweet potato cultivars, and these markers are expected to enhance molecular studies and improve sweet potato quality in the face of climate change.
Article Abstract
In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The lengths of the developed EST-SSR markers ranged from 100 to 499 bp (average length 238 bp). Their motif sequence types were varied, with most being dinucleotides and pentanucleotides, and the most commonly found motifs were CAGAAT (29.0%) and TCT (2.8%). Based on these SSR-containing sequences, 619 pairs of high-quality SSR primers were designed using WebSat and Primer3web. The total number of primers designed was 144. Polymorphism was evident in 82 EST-SSR markers among 20 Korean sweet potato cultivars tested and in 90 EST-SSR markers in the two parents of a mapping population, Yeseumi and Annobeny. In this study, the hexaploid sweet potato (2n = 6x = 90) EST-SSR markers were developed in the absence of full-sequence data. Moreover, by acting as a molecular tag for particular traits, the EST-SSR marker can also simultaneously identify information about the corresponding gene. These EST-SSR markers will allow the molecular analysis of sweet potato to be done more efficiently. Thus, we can develop high-quality sweet potato while overcoming the challenges from climate change and other unfavorable conditions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234531 | PMC |
| http://dx.doi.org/10.1007/s13205-016-0565-9 | DOI Listing |
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