A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars 'Peach ND', 'Peach Red', and 'Vivid Scarlet' under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764610 | PMC |
| http://dx.doi.org/10.1007/s13205-016-0396-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Using synthetic biology to express nitrogenase biosynthesis pathway in rice and to overcome barriers of nitrogenase instability in plant cytosol.
Trends Biotechnol
January 2025
College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address:
Engineering nitrogen fixation in cereals could reduce usage of chemical nitrogen fertilizers. Here, a nitrogenase biosynthesis pathway comprising 13 genes (nifB nifH nifD nifK nifE nifN nifX hesA nifV nifS nifU groES groEL) was introduced into rice by transforming multigene vectors and subsequently by sexual crossing between transgenic rice plants. Genome sequencing analysis revealed that 13 nif genes in F hybrid rice lines L12-13 and L8-17 were inserted at two loci on rice chromosome 1.
View Article and Find Full Text PDFIntranasally administrated fusion-inhibitory lipopeptides block SARS-CoV-2 infection in mice and enable long-term protective immunity.
Commun Biol
January 2025
CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, Ecole Normale Supérieure de Lyon, Lyon, France.
We have assessed antiviral activity and induction of protective immunity of fusion-inhibitory lipopeptides derived from the C-terminal heptad-repeat domain of SARS-CoV-2 spike glycoprotein in transgenic mice expressing human ACE2 (K18-hACE2). The lipopeptides block SARS-CoV-2 infection in cell lines and lung-derived organotypic cultures. Intranasal administration in mice allows the maintenance of homeostatic transcriptomic immune profile in lungs, prevents body-weight loss, decreases viral load and shedding, and protects mice from death caused by SARS-CoV-2 variants.
View Article and Find Full Text PDFGenome-wide identification of WRKY transcription factor genes in Euphorbia lathyris reveals ElWRKY48 as a negative regulator of phosphate uptake and ingenol biosynthesis.
Int J Biol Macromol
January 2025
Institute of Botany, Jiangsu Province, Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China; Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-cultivation and High-value Utilization of Chinese Medicinal Materials, Nanjing 210014, China. Electronic address:
WRKY transcription factors (TFs) play pivotal roles in regulating plant nutrient uptake, particularly phosphate (Pi) acquisition, and biosynthesis of secondary metabolites. Euphorbia lathyris, a significant medicinal plant with diverse pharmacological activities, lacks a systematic analysis of WRKY members and their functional roles. In this study, 58 ElWRKY genes were identified in the E.
View Article and Find Full Text PDFOverexpression of the Vitis amurensis Ca-binding protein gene VamCP1 in Arabidopsis thaliana and grapevine improves cold tolerance.
Physiol Plant
January 2025
College of Enology and Horticulture, Ningxia University/College of Modern Grape and Wine Industry/Ningxia Grape and Wine Research Institute/Engineering Research Center of Grape and Wine, Ministry of Education, Yinchuan, P.R. China.
Calcium ions (Ca) are important second messengers and are known to participate in cold signal transduction. In the current study, we characterized a Ca-binding protein gene, VamCP1, from the extremely cold-tolerant grape species Vitis amurensis. VamCP1 expression varied among organs but was highest in leaves following cold treatment, peaking 24 h after treatment onset.
View Article and Find Full Text PDFArabidopsis glycosyltransferase UGT86A1 promotes plant adaptation to salt and drought stresses.
Physiol Plant
January 2025
The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education; Shandong Key Laboratory of Precision Molecular Crop Design and Breeding; School of Life Sciences, Shandong University, Qingdao, China.
UDP-glycosyltransferases (UGTs) are the largest glycosyltransferase family developed during the evolution of the plant kingdom. However, their physiological significance in abiotic stress adaptation in land plants is largely unknown. In this study, we identified a UGT gene from Arabidopsis thaliana, UGT86A1, that was significantly induced by salt and drought stresses.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!