, a tetraploid wild relative of cultivated rice (family Poaceae), possesses a BBCC genome and contains genes that confer resistance to bacterial blight (BB) and white-backed (WBPH) and brown (BPH) plant hoppers. Based on the importance of this wild species, this study aimed to understand the phylogenetic relationships of with other species through an in-depth analysis of the composition and diversity of the chloroplast (cp) genome. The analysis revealed a cp genome size of 135,094 bp with a typical quadripartite structure and consisting of a pair of inverted repeats separated by small and large single copies, 139 representative genes, and 419 randomly distributed microsatellites. The genomic organization, gene order, GC content and codon usage are similar to those of typical angiosperm cp genomes. Approximately 30 forward, 28 tandem and 20 palindromic repeats were detected in the . cp genome. Comparison of the complete cp genome with another eleven species showed a high degree of sequence similarity and relatively high divergence of intergenic spacers. Phylogenetic analyses were conducted based on the complete genome sequence, 65 shared genes and gene showed same topologies and O. forms a single clade with parental . Thus, the complete . cp genome provides interesting insights and valuable information that can be used to identify related species and reconstruct its phylogeny.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339285PMC
http://dx.doi.org/10.3389/fpls.2017.00304DOI Listing

Publication Analysis

Top Keywords

complete genome
12
genome
8
chloroplast genome
8
species
5
complete
4
complete chloroplast
4
genome wild
4
wild rice
4
rice comparison
4
comparison species
4

Similar Publications

Targeted insertion of heterogenous DNA using Cas9-gRNA ribonucleoprotein-mediated gene editing in .

Bioengineered

December 2025

Department of BioMedical Bigdata (BK21) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.

Gene editing is emerging as a powerful tool for introducing novel functionalities in mushrooms. While CRISPR/Cas9-induced double-strand breaks (DSBs) typically rely on non-homologous end joining (NHEJ) for gene disruption, precise insertion of heterologous DNA in mushrooms is less explored. Here, we evaluated the efficacy of inserting donor DNAs (8-1008 bp) with or without homologous arms at Cas9-gRNA RNP-induced DSBs.

View Article and Find Full Text PDF

Complete genome sequence of LLZ14, a nitrogen-fixing and plant growth-promoting bacterium.

Microbiol Resour Announc

January 2025

Centre de Biotechnologies Végétales et Microbiennes, Biodiversité et Environnement, Faculty of Sciences, Mohammed V University in Rabat, Rabat, Morocco.

In this study, we present the complete genome of LLZ14, a nodule-forming bacterium isolated from root nodules with high plant growth-promoting abilities. This genome contains genes predicted to be involved in plant stress tolerance and growth promotion, including auxin production, phosphatase, and 1-aminocyclopropane-1-carboxylate deaminase.

View Article and Find Full Text PDF

Average nucleotide identity (ANI) is a widely used metric to estimate genetic relatedness, especially in microbial species delineation. While ANI calculation has been well optimized for bacteria and closely related viral genomes, accurate estimation of ANI below 80%, particularly in large reference data sets, has been challenging due to a lack of accurate and scalable methods. To bridge this gap, we introduce MANIAC, an efficient computational pipeline optimized for estimating ANI and alignment fraction (AF) in viral genomes with divergence around ANI of 70%.

View Article and Find Full Text PDF

Identification of near full-length human pegivirus type 2 (HPgV-2) genomes in blood donor samples co-infected with hepatitis C virus (HCV).

Microbiol Resour Announc

January 2025

Laboratory of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.

Human pegivirus (HPgV) identified from an HCV-infected plasma sample through nanopore metagenomics. The analysis revealed a nearly complete HPgV-2 genome. Phylogenetic analysis confirmed its classification within the HPgV-2 genotype, providing insights into viral co-infection dynamics.

View Article and Find Full Text PDF

This article reports on the complete genome of sp. strain Stari2, which was shown to have the ability to degrade carbon tetrachloride (CCl) in aerobic conditions. A single circular sequence of 6,310,573 bp, a GC content of 60.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!