Novel hemostatic agents based on gelatin-microbial transglutaminase mix.

Sci China Life Sci

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China.

Published: April 2017

AI Article Synopsis

  • Hemostasis poses significant challenges during surgeries and trauma treatment, as traditional methods can be ineffective and damaging to tissues.
  • Researchers developed a new hemostatic agent made from gelatin cross-linked with a novel microbial transglutaminase (mTGase), differing from existing ones, to mimic the blood coagulation process.
  • In tests on rat liver models, this new gelatin-mTGase sealant demonstrated comparable hemostatic effectiveness to a standard product (SURGIFLO) while showing enhanced adhesion and elasticity, marking it as a promising surgical solution.

Article Abstract

Hemostasis is a major challenge in surgical procedures and traumas. Conventional hemostatic methods have limited efficacy and may cause additional tissue damage. In this study, we designed a novel hemostatic agent based on the in situ gel formation of gelatin cross-linked by a novel microbial transglutaminase (mTGase), in which the amino acid sequences differed from commercial mTGases. The new hemostatic agent showed the same biochemical crosslinking chemistry as the final stages of the blood coagulation cascade while using gelatin as a "structural" protein (rather than fibrin) and a calcium-independent mTGase as the crosslinking catalyst (rather than factor XIIIa). In rat liver hemostasis models, the hemostatic agent not only showed a similar hemostatic effect as that of SURGIFLO (positive control), but also stronger adhesion strength and elasticity than SURGIFLO. Therefore, this biomimetic gelatin-mTGase mix hemostatic is a novel and effective surgical sealant.

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Source
http://dx.doi.org/10.1007/s11427-015-9019-xDOI Listing

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