A method for determining cannabinoids, Δ9-tetrahidrocannabinol (THC), 11-nor-9-carboxy-Δ-THC (THC-COOH) and 11-hidroxy-Δ-THC (THC-OH) in milk, liver and hemp seeds based on liquid chromatography tandem mass spectrometry has been optimized and validated. Analytes were extracted with methanol and the extracts cleaned-up by solid-phase extraction using Oasis HLB (60mg). The developed method was validated according to the Commission Decision 2002/657/EC. The decision limit (CCα) and detection capability (CCβ) ranged from 3.10-10.5ngg and 3.52-11.5ngg, the recoveries were 76-118% and matrix effect ranged from -17.8% to 19.9% in the three matrices studied. The method was applied to food samples obtaining positive results for THC in hemp seeds (average 0.82μgg) and three brands of junior formula milk at concentrations from 4.76 to 56.11ngg. The developed method was suitable achieving identification and quantification of cannabinoids in food matrices.
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| http://dx.doi.org/10.1016/j.foodchem.2017.01.128 | DOI Listing |
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