AI Article Synopsis
- In 32-cell stage embryos of Patella vulgata, one macromere interacts with micromeres, triggering differentiation into mesodermal stem cells.
- An extracellular matrix (ECM) formed by the micromeres is found between these cell types, containing glycoconjugates likely in the form of proteoglycans.
- Various staining techniques and lectin labeling indicate the ECM's specific characteristics, suggesting its crucial role in mesodermal cell induction.
Article Abstract
In 32-cell stage embryos ofPatella vulgata one of the macromeres contacts the animal micromeres, and as a result is induced to differentiate into the stem cell of the mesodermal cell line. In this study we show the presence of an extracellular matrix (ECM) between these two interacting cell types. The ECM appears to be formed by the micromeres during the 32-cell stage. Staining experiments with alcian blue and tannic acid indicate that in contains glycoconjugates, possibly in the form of proteoglycans. The characteristics of the ECM were examined further by fluorescein isothiocyanate (FITC)-lectin labelling. Of 17 lectins tested, concanavalin A (ConA), succinyl-ConA, LCH-B (Lens culinaris) and PEA (Pisum sativum) showed a positive labelling of the ECM. These results are in accordance with the electron microscopic data. The appearance of the ECM at this specific stage and place suggests that it might play an important role in the induction of the mesodermal cell line.
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| http://dx.doi.org/10.1007/BF02438960 | DOI Listing |
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