Allantoic endoderm of 3.5-day chick embryos was cultured in recombination with digestive-tract mesenchymes of 6-day chick embryos, and the differentiation of the endoderm was studied, with special attention being given to the appearance of brush-border (BB) antigens and sucrase. Irrespective of the origin of the associated digestive-tract mesenchymes, the allantoic endoderm differentiated into a columnar epithelium, expressing BB antigens and sucrase, and also into a BB antigen-negative pseudostratified or stratified epithelium of cuboidal or columnar cells with PAS or alcian blue staining in the apical portion or a BB antigen-negative stratified squamous epithelium. These results suggest that 3.5-day allantoic endoderm has the potency to differentiate into intestinal and cloacal epithelium.
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