Two RNA polymerases, enzyme A and enzyme B, were prepared fromPhysarum plasmodia. They are not located in the cytosol. Isolated nuclei, however, contain only a fraction of the total RNA polymerase activity: 1.5-19% depending on the nuclear preparation method.The level of RNA polymerase B shows little variation at developmental stages of growth and encystment, or in cysts and during germination, whereas RNA polymerase A displays a marked transient decrease in activity during the stationary growth phase before encystment.
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http://dx.doi.org/10.1007/BF00848103 | DOI Listing |
Plant Dis
January 2025
The University of Melbourne, Faculty of Science, School of Agriculture, Food and Ecosystem Sciences, Parkville, Victoria, Australia;
In Australia, pyrethrum (Tanacetum cinerariifolium) cultivation provides a significant portion of the global supply of natural insecticidal pyrethrins. However, crown and root rots, along with stunted plant growth and plant loss during winter, are significant issues affecting certain sites. Several isolates of the Fusarium oxysporum species complex (FOSC) have been identified as causal agents of crown and root rot in pyrethrum, highlighting these as key pathogens contributing to this decline.
View Article and Find Full Text PDFNeurochem Res
January 2025
Department of Neurology, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China.
Our aim was to evaluate the regulation of messenger RNAs (mRNAs) and biological pathways by long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) in ischemic stroke. We employed weighted gene co-expression network analysis (WGCNA) to construct two co-expression networks for mRNAs with circRNAs and lncRNAs, respectively, to investigate their association with ischemic stroke. We compared the overlap of mRNAs and biological pathways in the stroke-associated modules of the two networks.
View Article and Find Full Text PDFTrop Anim Health Prod
January 2025
Department of Agricultural Biotechnology, Faculty of Agriculture, Kırşehir Ahi Evran University, 40100, Kirsehir, Türkiye.
The present study was conducted on specific skeletal muscles of six weaned male kids from each of the Angora, Hair, Honamlı, and Kilis goat breeds. The relationships between the expression of myogenic factor 5 (Myf5) and myogenic factor 6 (Myf6) genes and muscle fibre characteristics were analysed. Muscle samples from the longissimus dorsi (LD) and semitendinosus (ST) were collected from six 90-day-old weaned male kids of each breed.
View Article and Find Full Text PDFMol Biol Rep
January 2025
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, People's Republic of China.
Background: Paeonia lactiflora Pall., a member of Paeoniaceae family, is a medicinal herb widely used in traditional Chinese medicine. Chloroplasts are multifunctional organelles containing distinct genetic material.
View Article and Find Full Text PDFMol Cancer Ther
January 2025
Tango Therapeutics (United States), Boston, United States.
Synthetic lethality approaches in BRCA1/2-mutated cancers have focused on poly(ADP-ribose) polymerase (PARP) inhibitors, which are subject to high rates of innate or acquired resistance in patients. Here, we used CRISPR/Cas9-based screening to identify DNA Ligase I (LIG1) as a novel target for synthetic lethality in BRCA1-mutated cancers. Publicly available data supported LIG1 hyperdependence of BRCA1-mutant cells across a variety of breast and ovarian cancer cell lines.
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