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Molecular characterization of an Akabane virus isolate from West Java, Indonesia. | LitMetric

Molecular characterization of an Akabane virus isolate from West Java, Indonesia.

J Vet Med Sci

Disease Investigation Center Subang, Directorate General of Livestock and Animal Health Services, Jl. Terusan Garuda Blok Werasari, RT.33/RW.11, Subang, West Java 41212, Indonesia.

Published: April 2017

AI Article Synopsis

  • The virus was identified using AKAV-specific RT-PCR techniques and detailed sequence analysis.
  • Phylogenetic analysis showed that this isolate (WJ-1SA/P/2014) is closely related to other AKAV isolates from Israel and Turkey, confirming it belongs to the AKAV genogroup Ib and marking the first recorded case of AKAV in Indonesia.

Article Abstract

We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate belongs to AKAV genogroup Ib. This is the first isolation of AKAV from Indonesia.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402201PMC
http://dx.doi.org/10.1292/jvms.17-0009DOI Listing

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