AI Article Synopsis
- The study focused on finding stable reference genes for normalizing qRT-PCR data in human cervical tissues affected by cancer and non-cancer states.
- PUM1, YWHAZ, and RPLP0 emerged as the top reference genes that maintained stable expression levels across various tissue types.
- The findings were analyzed based on factors like malignancy status, cancer type, and human papillomavirus type.
Article Abstract
This study aimed to identify the most stably expressed reference genes from a panel of 32 candidate genes for normalization of reverse transcription-quantitative real-time polymerase chain reaction data in cancerous and non-cancerous tissues of human uterine cervix. Overall, PUM1, YWHAZ, and RPLP0 were identified as the most stably expressed genes in paired cancerous and non-cancerous tissues. The results were further stratified by the state of malignancy of the tissues, histopathological type of the cancer, and the human papillomavirus-type.
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| http://dx.doi.org/10.1080/07357907.2017.1278767 | DOI Listing |
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