Characterization of a new badnavirus from Wisteria sinensis.

The complete genome sequence of a previously undescribed badnavirus isolated from a wisteria plant exhibiting mosaic and crinkle symptoms in Beijing, China, was determined. The circular double-stranded DNA genome of this virus was 7362 bp in size with four open reading frames (ORFs 1 to 4) on the plus strand. Sequence analysis showed that this virus shared the highest (69%) nucleotide (nt) sequence identity with pagoda yellow mosaic associated virus (PYMAV). In the RT-RNase H region of the ORF-3 encoded polyprotein, this virus shared 74% nt sequence identity with PYMAV. Phylogenetic analysis provided further evidence that the virus identified in this study is a member of a new species in the genus Badnavirus. The name wisteria badnavirus 1 (WBV1) is proposed for this new virus.