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| http://dx.doi.org/10.7554/eLife.26811 | DOI Listing |
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Two-fluid dynamics and micron-thin boundary layers shape cytoplasmic flows in early embryos.
Proc Natl Acad Sci U S A
October 2023
Department of Physics, École Normale Supérieure, Paris 75005, France.
Cytoplasmic flows are widely emerging as key functional players in development. In early embryos, flows drive the spreading of nuclei across the embryo. Here, we combine hydrodynamic modeling with quantitative imaging to develop a two-fluid model that features an active actomyosin gel and a passive viscous cytosol.
View Article and Find Full Text PDFTwo-fluid dynamics and micron-thin boundary layers shape cytoplasmic flows in early embryos.
bioRxiv
March 2023
École Normale Supérieure, 75005 Paris, France.
Cytoplasmic flows are widely emerging as key functional players in development. In early embryos, flows drive the spreading of nuclei across the embryo. Here, we combine hydrodynamic modeling with quantitative imaging to develop a two-fluid model that features an active actomyosin gel and a passive viscous cytosol.
View Article and Find Full Text PDFUsing FCS to accurately measure protein concentration in the presence of noise and photobleaching.
Biophys J
October 2021
Department of Physics and Astronomy, McMaster University, Hamilton, Ontario, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada. Electronic address:
Quantitative cell biology requires precise and accurate concentration measurements, resolved both in space and time. Fluorescence correlation spectroscopy (FCS) has been held as a promising technique to perform such measurements because the fluorescence fluctuations it relies on are directly dependent on the absolute number of fluorophores in the detection volume. However, the most interesting applications are in cells, where autofluorescence and confinement result in strong background noise and important levels of photobleaching.
View Article and Find Full Text PDFBicoid-Dependent Activation of the Target Gene hunchback Requires a Two-Motif Sequence Code in a Specific Basal Promoter.
Mol Cell
September 2019
Department of Biology, New York University, 100 Washington Square East, New York, NY 10003, USA. Electronic address:
In complex genetic loci, individual enhancers interact most often with specific basal promoters. Here we investigate the activation of the Bicoid target gene hunchback (hb), which contains two basal promoters (P1 and P2). Early in embryogenesis, P1 is silent, while P2 is strongly activated.
View Article and Find Full Text PDFDynamic maternal gradients and morphogenetic networks in Drosophila early embryo.
Biosystems
November 2018
Mathematical Biology and Bioinformatics Laboratory, Peter the Great St. Petersburg Polytechnic University, Polytekhnicheskaya 29, 195251, St. Petersburg, Russia. Electronic address:
Here, we review the latest publications on dynamics and interpretation of morphogen gradients in Drosophila early embryo. The instructive cues provided by these gradients are further interpreted by target genes to enable correct cell fate specification. Moreover, recent studies point on the dynamic and active input from gradients themselves.
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