AI Article Synopsis
- There are 14 defined cluster-designated (CD) antigens that characterize myelomonocytic cells, with 5 being particularly useful for myeloid leukemia classification (CD13, CD14, CD15, CD33, CD36).
- Despite their potential, monoclonal antibodies for these CD antigens showed low reactivity with myeloblastic leukemia cells.
- Monoclonal antibodies against myeloperoxidase were developed, proving to be highly sensitive for diagnosing acute myeloid leukemia, surpassing traditional cytochemical stains.
Article Abstract
Since the last workshop on human leukocyte differentiation antigens, there are 14 well defined cluster-designated (CD) antigens which characterize myelomonocytic cells. Of these, 5 are potentially useful for myeloid leukemia typing (i.e. CD13, CD14, CD15, CD33, CD36) because they are cell lineage-specific and also expressed on immature cells. However, the reactivity of monoclonal anti-CD antibodies, directed against these antigens, with myeloblastic leukemia cells was found to be quite low. We produced monoclonal antibodies against myeloperoxidase. These antibodies react also with promyeloperoxidase, synthesized in HL-60 cell line cells. Monoclonal antimyeloperoxidase was found to be the most sensitive reagent to diagnose acute myeloid leukemia, even more sensitive than cytochemical stains (Sudan black, myeloperoxidase).
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| http://dx.doi.org/10.1159/000205900 | DOI Listing |
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