Cattle are a primary reservoir of enterohemorrhagic Escherichia coli (EHEC), and contaminated beef products are a source of human infections. The U.S. Department of Agriculture Food Safety and Inspection Service declared seven EHEC serogroups (O26, O45, O103, O111, O121, O145, and O157) as adulterants in raw ground beef. Sampling a large number of animals for EHEC surveillance or evaluations of EHEC-focused preharvest interventions requires a convenient and robust sampling method. We evaluated the diagnostic performance of rectoanal mucosal swab (RAMS) for the detection of the top seven EHEC serogroups. Paired fecal grab (FG) and RAMS samples were collected from 176 beef cattle and tested using the NeoSEEK Shiga toxin-producing E. coli (STEC) confirmation method. The prevalence of virulence-associated genes (stx, stx, stx, eae, and nleB) was higher in RAMS than in FG samples. The results of the two methods had poor agreement, as indicated by kappa statistics, for the detection of the seven serogroups. When FG and RAMS results were combined for comparison, RAMS was more sensitive than FG for the detection of serogroups O103 (82% versus 39%), O157 (75% versus 67%), and O45 (79% versus 73%) with similar sensitivity for the detection of serogroup O145 (67%). Serogroups O111 and O121 were detected from one and two samples, respectively, by FG and were not detected by RAMS. Serogroup O26 was not detected with either method. RAMS appears to be equivalent or superior to FG sampling for detection of the top seven EHEC serogroups in the feces of beef cattle with the NeoSEEK STEC confirmation test.
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http://dx.doi.org/10.4315/0362-028X.JFP-16-435 | DOI Listing |
J Comp Pathol
January 2025
Setor de Patologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Although peritonitis is highly prevalent in cattle, there have been only limited studies on the pathology of this condition. We describe the gross and histological aspects of primary and secondary peritonitis in cattle based on necropsy reports of 46 cases. Twenty-six were female (26/46; 56.
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January 2025
Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP 18618-970, Brazil.
We evaluated the effects of breed and mineral source on heifer performance during periods of nutrient restriction and grazing. On day -7, ½ Angus × ½ Nelore (ANE) and Nelore (NE) heifers (12 heifers per breed; body weight, BW = 264 ± 35 kg; age = 15 ± 1 mo) were assigned to individual drylot pens to receive ad libitum Tifton 85 (Cynodon sp.) hay and white salt for 7 days.
View Article and Find Full Text PDFElife
January 2025
Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Canada.
Several areas of the world suffer a notably high incidence of Shiga toxin-producing . To assess the impact of persistent cross-species transmission systems on the epidemiology of O157:H7 in Alberta, Canada, we sequenced and assembled O157:H7 isolates originating from collocated cattle and human populations, 2007-2015. We constructed a timed phylogeny using BEAST2 using a structured coalescent model.
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January 2025
Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
The aim of this study was to investigate whether Trypanosoma vivax is transmitted via rectal palpation in cattle, using the same glove with different blood scores, from an animal with an acute infection of this protozoan. In addition, the efficacy of iodine as disinfectant, together with water with or without the presence of feces, to prevent the transmission of T. vivax in cattle during the rectal palpation process was evaluated in the laboratory and animals.
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January 2025
Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, China. Electronic address:
The differentiation and lipid metabolism of preadipocytes are crucial processes in IMF deposition. Studies have demonstrated that SIRT4 plays essential roles in energy metabolism and redox homeostasis, with its expression being coordinately regulated by multiple transcription factors associated with energy and lipid metabolism. In this study, the findings of multiple omics analysis reveal that SIRT4 significantly up-regulates the expression of genes involved in adipogenesis and enhances the differentiation and lipid deposition of bovine preadipocytes.
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