Virus-specific RNA was detected in FLK cells producing bovine leukemia virus (BLV) by cDNA hybridization in situ. For calculation of the virus-specific RNA copy number in infected cells hybridization of 3H-cDNA BLV with the total cellular RNA in solution was used. FLK cells contain approximately 600 copies of virus-specific RNA per cell. Individual FLK cells producing BLV were identified by hybridization in situ. A successful identification of viral RNA in cells by hybridization in situ is possible if cells contain more than 100 copies of viral transcripts.
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BNT162b2 mRNA vaccine elicits robust virus-specific antibodies but poor cross-protective CD8 memory T cell responses in adolescents with type 1 diabetes.
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Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, 70101, Taiwan; Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 70101, Taiwan. Electronic address:
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