The phytohormone abscisic acid (ABA) is essential in plant responding to biotic and abiotic stresses. Although ABA signaling model is well established in , ABA receptor PYL family and clade A PP2C subfamily are not yet characterized in monocot model plant . In this study, we identified 12 and 8 clade A from genome and successfully cloned 12 and 7 clade A . Bioinformatic and expression analyses showed that most of the identified genes respond to several signal molecules and abiotic stresses. Protein-protein interaction analysis revealed that many BdPYLs and BdPP2CAs participate in the classic ABA-PYL-PP2C-SnRK2 signaling pathway. A clade A PP2C, designated BdPP2CA6, interacted with BdPYL11 in the absence of ABA and localized in nucleus. Most clade A PP2C members from showed negatively regulation in ABA signaling pathway, whereas -overexpression transgenic showed ABA hypersensitive phenotype, resulting in enhanced stomatal closure and salinity tolerance. Our results indicate that positively regulates ABA and stress signal pathway in transgenic plant seedlings.
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| http://dx.doi.org/10.3389/fpls.2017.00264 | DOI Listing |
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