AI Article Synopsis
- A study was conducted to examine the expression and potential roles of Gab3 in human glioma, showing elevated levels in glioma tissues and cells compared to normal tissues.
- Gab3 knockdown through shRNA/siRNA significantly impeded Akt activation and proliferation in glioma cells, while its overexpression enhanced these processes.
- In vivo experiments revealed that tumors with reduced Gab3 expression showed inhibited growth and decreased Akt activation, indicating that Gab3 plays a critical role in glioma development.
Article Abstract
This current study tested expression and potential biological functions of Gab3 in human glioma. Gab3 mRNA and protein expression was significantly elevated in human glioma tissues and glioma cells. Its level was however low in normal brain tissues and primary human astrocytes. In both established (U251MG cell line) and primary human glioma cells, Gab3 knockdown by shRNA/siRNA significantly inhibited Akt activation and cell proliferation. Reversely, forced Gab3 overexpression in U251MG cells promoted Akt activation and cell proliferation. In vivo, the growth of U251MG tumors in nude mice was inhibited following expressing Gab3 shRNA. Akt activation in cancer tissues was also suppressed by Gab3 shRNA. Together, we conclude that Gab3 overexpression in human glioma mediates Akt activation and cancer cell proliferation.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349442 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0173473 | PLOS |
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