In this work, the ability of asymmetrical flow field-flow fractionation (AF4) coupled with UV-vis, multiangle light scattering (MALS), and quasi-elastic light scattering (QELS) (AF4-UV-MALS-QELS) for monitoring aggregation of components of egg yolk plasma was evaluated. The effect of external factors (i.e., pH, storage conditions, freezing and heat treatments) on the egg yolk plasma was studied. The results reveal that the aggregation mechanism of components of egg yolk plasma during heat and freezing treatment is different. The results suggest that the low density lipoproteins (LDLs) in egg yolk plasma undergo a 'clusters-fusion-gel' process under heat treatment at pH10. The alkaline conditions promote the formation of LDL aggregates. Also, storage conditions play a role in the formation of LDL aggregates. It was found that the hen eggs stored for 7days at room temperature contain less aggregate than those stored at 4°C for the same period. The combination of AF4 with online MALS-QELS provided conformational information in terms of the shape and size distribution of LDL aggregates. AF4-UV-MALS-QELS was proved to be a rapid and gentle method for the separation and characterization of egg yolk plasma aggregates.
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http://dx.doi.org/10.1016/j.foodres.2017.01.025 | DOI Listing |
Poult Sci
December 2024
Department of Poultry Science, College of Agriculture, Tarbiat Modares University, Tehran, Iran 14115336.
This study was conducted to evaluate the effects of E.coli Nissle 1917 (EcN) on immune responses, blood parameters, oxidative stress, egg quality, and performance of laying Japanese quail. A total of one-hundred day-old quail chicks were assigned to 1 of 4 treatments based on probiotic concentration: 1 (0 CFU/mL; control), 2 (10 CFU/mL), 3 (10 CFU/mL), and 4 (10 CFU/mL).
View Article and Find Full Text PDFTrop Anim Health Prod
January 2025
Federal Institute of Education, Science, and Technology of Amazonas, Downtown Campus, Manaus, Amazonas, Brazil.
This study evaluated the effects of incorporating biological silage from tambaqui (Colossoma macropomum) by-products (BST) on the performance, hematological and plasma biochemical parameters, and egg quality (physical, proximate composition and sensory characteristics) of older commercial hens. The BST was prepared by ensiling tambaqui by-products with lactic acid bacteria, cassava trimmings, and preservatives, producing a nutrient-rich, high-protein feed ingredient. One hundred and twenty Hisex Brown hens (83 weeks old) were divided into five treatment groups, each receiving diets with 0, 1, 2, 3, or 4% BST.
View Article and Find Full Text PDFFront Vet Sci
December 2024
Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing, China.
The purpose of this study was to investigate the effects of dietary supplementation of compound probiotics on the performance, egg quality, biochemical parameters and intestinal morphology of laying hens. A total of 180 healthy 200-day-old Hyline Brown laying hens with similar initial laying rate (87.5% ± 0.
View Article and Find Full Text PDFFoods
December 2024
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China.
Chicken yolk immunoglobulin (IgY) is a natural immunologically active antibody extracted from egg yolk and can be used as a natural dietary supplement for the treatment of inflammation and damage to the intestines. In our study, IgY was embedded in a double emulsion (W/O/W; DE) to explore the therapeutic effect of the embedded IgY on Lipopolysaccharide (LPS)-induced jejunal injury in mice. The results showed that W/O/W-embedded IgY as a dietary supplement (IgY + DE) attenuated LPS-induced damage to mouse small intestinal structures and protected the integrity of the jejunal mucosal barrier.
View Article and Find Full Text PDFFoods
December 2024
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Due to imbalanced demand favoring egg whites, the egg industry faces a surplus of egg yolk, limiting overall growth. This study designed a feasible process for the crude extraction of livetins and phosvitin (PV) and revealed the related separation mechanisms. Our method utilized a 1:9 egg yolk dilution at pH 6.
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