Assessment and Optimizations of Candida albicans Biofilm Assays.

Antimicrob Agents Chemother

Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced, Merced, California, USA

Published: May 2017

biofilms have a significant medical impact due to their rapid growth on implanted medical devices, their resistance to antifungal drugs, and their ability to seed disseminated infections. Biofilm assays performed allow for rapid, high-throughput screening of gene deletion libraries or antifungal compounds and typically serve as precursors to studies. Here, we compile and discuss the protocols for several recently published biofilm assays. We also describe improved versions of these protocols as well as novel assays. Finally, we consider some of the advantages and disadvantages of these different types of assays.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404589PMC
http://dx.doi.org/10.1128/AAC.02749-16DOI Listing

Publication Analysis

Top Keywords

biofilm assays
12
assays
5
assessment optimizations
4
optimizations candida
4
candida albicans
4
albicans biofilm
4
assays biofilms
4
biofilms medical
4
medical impact
4
impact rapid
4

Similar Publications

Genotypic diversity and virulence factors of Klebsiella pneumoniae in a North Indian tertiary care hospital.

BMC Infect Dis

December 2024

Lab Services and Infection Control; Chief, Education and Research, Artemis Hospitals, Sector-51, Gurugram, Haryana, India.

Klebsiella pneumoniae, a pathogen of concern worldwide can be classified as classical K. pneumoniae (cKp) and Hypervirulent K. pneumoniae (HvKp).

View Article and Find Full Text PDF

Background: Pseudomonas aeruginosa is one of the leading causes of nosocomial infections and the most common multidrug-resistant pathogen. This study aimed to determine antimicrobial resistance patterns, biofilm-forming capacity, and associated factors of multidrug resistance in P. aeruginosa isolates at two hospitals in Addis Ababa, Ethiopia.

View Article and Find Full Text PDF

Crystal-violet staining, microscopy with image analysis, and quantitative PCR (qPCR) were compared to examine biofilm dynamics. Biofilms of 30 polycultures comprising 15 bacterial species were monitored for 14 days. Collectively, qPCR (representing population) revealed a different growth pattern compared to staining (biomass) and microscopy (colonization): biomass and colonization gradually increased over time, whereas population increased rapidly for the first seven days and leveled off.

View Article and Find Full Text PDF

Deciphering the key role of biofilm and mechanisms in high-strength nitrogen removal within the anammox coupled partial S-driven autotrophic denitrification system.

Bioresour Technol

December 2024

Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Industry and Information Technology, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China; Engineering Research Centre of Chemical Pollution Control, Ministry of Education, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China. Electronic address:

Anammox coupled partial S-driven autotrophic denitrification (PSAD) technology represents an innovative approach for removing nitrogen from wastewater. The research highlighted the crucial role of biofilm on sulfur particles in the nitrogen removal process. Further analysis revealed that sulfur-oxidizing bacteria (SOB) are primarily distributed in the inner layer of the biofilm, while anammox bacteria (AnAOB) are relatively evenly distributed in inner and outer layers, with Thiobacillus and Candidatus Brocadia being the dominant species, respectively.

View Article and Find Full Text PDF

Chlorhexidine-loaded microneedles for treatment of oral diseases.

Int J Pharm

December 2024

Department of Pharmaceutical Engineering, Azrieli College of Engineering Jerusalem, Jerusalem 9103501, Israel. Electronic address:

Chlorhexidine (CHX) is a gold standard therapeutic agent against clinical oral pathogens. However, its oral use is limited due to unpleasant taste, alteration in taste buds, staining of teeth and mucous membranes. Therefore, CHX-loaded PLGA microneedles (MNs) were fabricated for local and controlled release in the oral cavity, using a casting mold method.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!