AI Article Synopsis
- - This study investigated the effectiveness of an ethanol extract and its fractions from a plant against leishmania parasites, focusing on their toxicity levels and selectivity index.
- - The ethanol extract was made by soaking powdered stems and was further separated into fractions; lapachol was extracted and purified for testing.
- - Results showed that the ethyl acetate fraction had activity against promastigotes without being toxic, while lapachol was selectively effective against amastigotes, indicating potential for future treatments.
Article Abstract
This study aimed to evaluate the leishmanicidal activity of ethanol extract, fractions, and isolated substance from against . Furthermore, this activity was related to cytotoxicity, and the selectivity index was determined. The ethanol extract was obtained by maceration of the stem powder, and the extract was subjected to fractionation on chromatographic column. The lapachol was obtained by acid base extraction followed by purification in chromatographic column. The antipromastigote activity and cytotoxicity tests were carried out by the cell viability method (MTT). Modified THP-1 cells were infected with promastigotes and treated for 24 h with different concentrations of the extract, fractions, and lapachol. The ethanol extract, dichloromethane, and ethyl acetate fractions were not active against promastigotes (IC > 200 g/mL) or cytotoxic (CC > 500 g/mL), and the selectivity index (SI) was greater than 2.5. The ethyl acetate fraction was active only in promastigotes; it is not cytotoxic (CC > 500 g/mL, SI > 5). The lapachol was selectively active only against amastigote (IS > 2.5, CC > 500 g/mL). In summary, lapachol and ethyl acetate fraction are promising against amastigote and promastigote forms, respectively.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5329664 | PMC |
| http://dx.doi.org/10.1155/2017/8074275 | DOI Listing |
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