Granulosa cells from follicles of different sizes from Booroola x Merino ewes which were homozygous (FF), heterozygous (F+) or non-carriers(++) of a fecundity gene were obtained 0-48 h after cloprostenol injection on Day 10 of the oestrous cycle. The highest mean amounts of cAMP produced by the cells did not differ between the genotypes. However, in the ++ ewes it was attained by cells from follicles greater than or equal to 5 mm in diameter, whereas in F+ and FF ewes it was attained by cells from follicles 3-4.5 mm in diameter. Cells from 1-2.5-mm diameter follicles of FF ewes were more sensitive to FSH and LH than were corresponding cells from F+ or ++ ewes. Granulosa cells from greater than or equal to 5 mm diameter follicles of ++ ewes 12-24 h after injection of cloprostenol had a lower mean response to FSH and LH than did cells obtained 0-6 or 36-48 h after cloprostenol. No such effect of time was evident for cells from any size of follicles obtained from F+ or FF ewes. In 1-2.5-mm diameter follicles, the mean aromatase activity of granulosa cells from ++ and F+ ewes was similar, but significantly lower than that of cells from FF ewes. In 3-4.5 mm diameter follicles, the mean aromatase activity of cells from F+ and FF ewes was similar, and significantly higher than that of cells from ++ ewes. For all 3 genotypes, there was a significant positive relationship between FSH or LH stimulation of granulosa cell cAMP production and cellular aromatase activity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1530/jrf.0.0810395 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Uterine histomorphological and immunohistochemical investigation during the follicular phase of estrous cycle in Saidi sheep.
BMC Vet Res
January 2025
Theriogenology Department, Faculty of Veterinary Medicine, New-Valley University, New Valley, 725211, Egypt.
Background: Saidi sheep are one of the most important farm animals in Upper Egypt, particularly in the Assiut governorate. Since they can provide meat, milk, fiber, and skins from low-quality roughages, sheep are among the most economically valuable animals bred for food in Egypt. Regarding breeding, relatively little is known about the Saidi breed.
View Article and Find Full Text PDFScabiosa artropurperea var.maritima aqueous extracts improve in vivo fertility parameters and in vitro granulosa cell steroidogenesis in Ewe.
Theriogenology
December 2024
CNRS, INRAE, Université de Tours, PRC, Nouzilly, France. Electronic address:
Scabiosa artropurperea, a member of the Dipsacaceae family and Scabiosa genus, is renowned for its medicinal properties. In the present study, we investigated the impact of Scabiosa artropurperea aqueous extract (AES) on the in vivo reproductive functions in Queue Fine de l'Ouest ewes, and on in vitro ovine granulosa cells. Ewes were synchronized for 14 days with intra-vagina progesterone (P4) devices (FGA, 20 mg) and divided into four groups receiving daily oral doses of 0, 1, 2, and 4 mg of AES/kg Live Body Weight (LBW), respectively.
View Article and Find Full Text PDFProlactin Inhibition Promotes Follicle Recruitment by Increasing Expression in Ewes During the Estrus Stage.
Animals (Basel)
December 2024
College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Prolactin (PRL) plays a key role in the growth and ovulation of animal follicles, but its impact on follicular recruitment in ewes remains uncertain. In this study, a total of sixteen healthy ewes (Hu sheep, aged 2-3 years, with continuous reproduction and housed separately), matched for parity and weight (52.98 ± 0.
View Article and Find Full Text PDFRad-mediated inhibition of Ca1.2 channel activity contributes to uterine artery haemodynamic adaptation to pregnancy.
J Physiol
December 2024
Lawrence D. Longo, MD Center for Perinatal Biology, Department of Basic Sciences, Loma Linda University School of Medicine, Loma Linda, CA, USA.
The striking increase of uterine blood flow during pregnancy is essential for normal fetal development as well as for cardiovascular well-being of the mother. Yet, the underlying mechanisms of pregnancy-mediated vasodilatation of the uterine artery are not fully understood. In this study, we test the hypothesis that Rad, a monomeric G protein, is a novel regulatory mechanism in inhibiting Ca1.
View Article and Find Full Text PDFAnalysis of differential expression of hair follicle tissue transcriptome in Hetian sheep undergoing different periodic changes.
PeerJ
November 2024
College of Animal Science, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Background: This study provides new information on long non-coding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in the hair follicles of Hetian sheep the sequencing and analysis of the transcriptome of skin hair follicles during three periods of periodicity change. This is important for improving the quality of carpet wool, providing a preliminary basis for further research on the targeting relationship of these mRNAs and their target genes, and providing a scientific basis for marker-assisted selection of Hetian sheep.
Methods: The periodic variation of anagen (P I, May, = 3), catagen (p II, October, = 3), and telogen (p III, January, = 3) of the skin hair follicle tissue of three Hetian sheep ewes were selected.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!