Model systems were chosen in an attempt to mimic the proton exchange environment of an agonist nitrogen in an opiate-receptor interaction. The two model systems studied were an ammonium: 18-crown-6 ether complex and a quinuclidine-trifluoroacetic acid ion pair. Each system was examined for their effects on 15N NMR INEPT enhancements. Both models were found to retard proton exchange dynamics, as observed by increased enhancements relative to free ions in neutral aqueous solutions. These results suggest that the confinement of a protonated nitrogen, such as that expected in receptor binding, may alter exchange dynamics to favor INEPT enhancements, while unbound agonists would remain unenhanced. As a result, 15N NMR INEPT enhancements from a solution of receptor subtypes with an appropriate 15N-labeled agonist may present a means of exploring the dynamics of direct opiate-receptor interactions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jps.2600761013 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prevalence of SARS-CoV-2 antibodies at the University hospital Heidelberg and correlation with SARS-CoV-2 incidence.
Heliyon
November 2024
Heidelberg University, Department of Infectious Diseases, Virology, Heidelberg, Germany.
Background: Since the onset of the SARS-CoV-2 pandemic in late 2019, many studies suggest that actual infection rates may far exceed reported cases. Therefore, this study provides a comprehensive overview of anti-SARS-CoV-2 antibody measurements and their significance in epidemiological surveillance, especially regarding the true extent of SARS-CoV-2 infections, allowing more detailed insights into the dynamics of the pandemic.
Methods: Antibodies were measured using the Elecsys® Anti-SARS-CoV-2 assay for the nucleocapsid (N) protein and the SARS-CoV-2 IgG (sCOVG) assay for the spike (S) protein.
Integrated effects of furrow irrigation systems and irrigation regimes on water productivity of onion (Allium L.) crop in a semi-arid area.
Heliyon
November 2024
Shire Institute of Technology, Aksum University, Shire, Ethiopia.
Dwindling water resources with inept field water management impends sustainable agricultural production in semi-arid areas of Ethiopia. Thus, the adaption and adoption of improved irrigation water running lines are indispensable and imperative to recuperate water productivity in areas where water resources are inadequate. The ground experiments were pointed to elucidate the weight of conventional, alternate, and fixed furrow irrigation systems under irrigation levels of 100 %, 70 %, and 40 % of crop evapotranspiration (ETc) on onion growth, yield, water productivity, and economic return in Alage, Central, Ethiopia.
View Article and Find Full Text PDFOvercoming the Challenges of Hyperpolarizing Substrates with Parahydrogen-Induced Polarization in an MRI System.
Chemistry
October 2024
Division of Medical Physics, Department of Diagnostic and Interventional Radiology, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Killianstrasse 5a, 79106, Freiburg, Germany.
Hyperpolarization of C nuclei in biomolecules and their administration as imaging agents enables in-vivo monitoring of metabolism. This approach has demonstrated potential for deriving imaging biomarkers for cancer detection, differentiation, and therapy efficacy assessment. The in situ generation of polarized substrates using a permanent addition of parahydrogen to an unsaturated precursor inside the bore of an MRI system used for subsequent imaging circumvents the need for a dedicated external polarizer.
View Article and Find Full Text PDFHeteronuclear Polarization Transfer under Steady-State Conditions: The INEPT-SSFP Experiment.
J Phys Chem Lett
October 2024
Department of Chemical and Biological Physics, Weizmann Institute, 7610001 Rehovot, Israel.
NMR finds a wide range of applications, ranging from fundamental chemistry to medical imaging. The technique, however, has an inherently low signal-to-noise ratio (SNR)─particularly when dealing with nuclei having low natural abundances and/or low γs. In these cases, sensitivity is often enhanced by methods that, similar to INEPT, transfer polarization from neighboring Hs via -couplings.
View Article and Find Full Text PDFMAS NMR experiments of corynebacterial cell walls: Complementary H- and CPMAS CryoProbe-enhanced C-detected experiments.
J Magn Reson
July 2024
Institute of Science and Technology Austria, Am Campus 1, Klosterneuburg, 3400, Austria. Electronic address:
Bacterial cell walls are gigadalton-large cross-linked polymers with a wide range of motional amplitudes, including rather rigid as well as highly flexible parts. Magic-angle spinning NMR is a powerful method to obtain atomic-level information about intact cell walls. Here we investigate sensitivity and information content of different homonuclear CC and heteronuclear HN, HC and NC correlation experiments.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!