We constructed a synthetic oligonucleotide corresponding to the previously proposed consensus binding site for the Fur protein, a central iron-regulatory protein of Escherichia coli. When this oligonucleotide was introduced at the start of transcription of an operon fusion between the ompF promoter and the lacZ structural gene, beta-galactosidase activity became iron regulated. This consensus sequence is sufficient to function as an operator site for the binding of Fur protein in vivo.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC210760 | PMC |
| http://dx.doi.org/10.1128/jb.170.2.1015-1017.1988 | DOI Listing |
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