AI Article Synopsis
- This study demonstrates the potential of IgY antibody-based immunoassays to detect antibiotic residues in food derived from animals.
- IgY antibodies were developed by immunizing chickens with full antigens of gentamicin and kanamycin, and the antibodies' effectiveness was tested using FPIA and ic-ELISA methods.
- Results showed that ic-ELISA had a lower limit of detection for both antibiotics compared to FPIA, indicating it may be a more effective method for detecting antibiotic residues.
Article Abstract
Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC of FPIA and ic-ELISA based anti-Gent IgY were 7.70±0.6μg/mL and 0.32±0.06μg/mL, respectively. The IC of FPIA and ic-ELISA based anti-Kana IgY were 7.97±0.9μg/mL and 0.15±0.01μg/mL. The limits of detection (LOD, IC) for FPIA based anti-Gent/Kana IgY were 0.17 and 0.007μg/mL, respectively. The LOD for ic-ELISA were both 0.001μg/mL. These results indicated that the ic-ELISA might more suitable for antibiotic residues detection than FPIA.
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| http://dx.doi.org/10.1016/j.foodchem.2017.01.058 | DOI Listing |
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Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA.
Food Chem
July 2017
College of Veterinary Medicine, Northwest A&F University, Yangling, 712100 Shaanxi, China; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China. Electronic address:
Article Synopsis
- This study demonstrates the potential of IgY antibody-based immunoassays to detect antibiotic residues in food derived from animals.
- IgY antibodies were developed by immunizing chickens with full antigens of gentamicin and kanamycin, and the antibodies' effectiveness was tested using FPIA and ic-ELISA methods.
- Results showed that ic-ELISA had a lower limit of detection for both antibiotics compared to FPIA, indicating it may be a more effective method for detecting antibiotic residues.
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