The effects of external and internal diffusions of pyridine on its biodegradation rate in a bioreactor with a stationary basket bed of immobilized Bacillus spp. cells have been investigated for various biocatalyst diameters and thicknesses of the basket bed, considering the adapted Haldane kinetic model for substrate inhibition. Due to the very low values of pyridine mass flow inside the biocatalyst particles, the 'biological inactive region' appeared mainly near the particles' centre. This region is extended up to 38.5% from the overall volume of each studied size of the biocatalysts, increasing at higher biocatalyst size and basket bed width. Compared to the system containing free Bacillus spp. cells, the basket configuration of packed bed led to the reduction of biodegradation rate up to 82 times, similar to the mobile bed or column packed bed. The cumulated analysis of the influences of the studied factors allowed concluding that the optimum diameter of biocatalysts is 3 mm.
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