The Crystal Diagnostics (CDx) Xpress S Kit is a rapid-screening assay for Salmonella spp. in whole raw tomatoes, whole chicken carcasses, raw ground beef, raw beef trim, and whole liquid pasteurized eggs with citric acid when present at levels of 1 CFU/portion size. The Xpress S system comprises an automatic CDx Xpress Reader and a single-use CDx BioCassette that incorporates antibody-coupled microspheres and liquid crystal for the selective identification of the intended microbe. In internal evaluations, the CDx Xpress S Kit detected all 142 Salmonella strains tested, including non-enterica subspecies, and excluded all non-Salmonella species assayed. Method-developer studies, as well as a third-party evaluation, demonstrated that 15 h single-stage enrichment permits rapid detection equivalent to the U.S. Department of Agriculture and U.S. Food and Drug Administration reference methods. The results demonstrate that the CDx Xpress S Kit is one of the fastest, most sensitive, and most accurate methods for detecting Salmonella in food matrixes.
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http://dx.doi.org/10.5740/jaoacint.17-0035 | DOI Listing |
Plant Dis
July 2024
University of Ljubljana, Biotechnical faculty, Agronomy Department, Jamnikarjeva 101, Ljubljana, Slovenia, 1000.
Apple hammerhead viroid (AHVd, Pelamoviroid, Avsunviroidae) is one of the five viroids infecting apples. It has been identified on all continents except Australia since its viroid nature was confirmed (DiSerio et al. 2018; CABI and EPPO 2022).
View Article and Find Full Text PDFSaudi J Med Med Sci
April 2024
Department of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Background: Viral infection is responsible for the majority of respiratory tract infections (RTI). This retrospective study evaluates the advantages of using the multiplex Xpert Xpress CoV-2/Flu/RSV plus kit in laboratory diagnosis of RTI caused by the most related viruses.
Materials And Methods: Data were obtained from medical records between November 2021 and May 2023 for any sample tested using either the Xpert Xpress Flu/RSV kit, Xpert Xpress Flu kit, Xpert Xpress SARS-CoV-2 kit, and Xpert Xpress CoV-2/Flu/RSV plus kit.
J Virol Methods
May 2024
Research and Innovation Unit. Hospital Universitario Costa del Sol, Autovía A-7, km 187, Málaga, Marbella 29603, Spain; Department of Surgical Specialties, Biochemistry and Immunology, Faculty of Medicine, University of Málaga. Málaga, Spain.
Reverse transcription polymerase chain reaction (RT-PCR) tests are commonly utilized in commercial settings but pose challenges due to labor-intensive procedures and extended response times during peak demand. In contrast, real-time fluorescence and isothermal amplification assays using Crossing Priming Amplification (CPA) offer faster genetic material analysis, eliminate subjectivity, and require less manipulation and personnel training. This study aimed to validate the EasyNAT SARS-CoV-2 Assay, a diagnostic kit based on CPA, using oral and nasopharyngeal samples.
View Article and Find Full Text PDFBackground: Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), can be diagnosed using rapid real-time polymerase chain reaction (PCR), real-time reverse transcription PCR (rRT-PCR), or rapid antigen testing. Among these, rRT-PCR is considered the gold standard assay. The Xpert Xpress SARS-CoV-2 assay is a rapid, real-time PCR test; approved by the Korean Disease Control and Prevention Agency in 2020.
View Article and Find Full Text PDFMed Sci Monit
January 2024
Department of Pediatrics, Ministry of National Guard Health Affairs, Dammam, Saudi Arabia.
BACKGROUND Childhood febrile seizures occur between 5 months and 6 years of age in children without a previous history of seizure and are associated with high temperature in the absence of intracranial infection. This retrospective study identified 71 children aged 6 months to 5 years with febrile seizures between 2017 and 2021 at a single center in Saudi Arabia and aimed to identify an association between common respiratory virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. MATERIAL AND METHODS Pediatric nasopharyngeal specimens were tested using a multiplex PCR respiratory panel detecting human coronaviruses (NL63, 229E, OC43, HKU1), influenza A/B, human adenovirus, parainfluenza viruses 1-4, respiratory syncytial virus, human metapneumovirus, rhinovirus/enterovirus, Middle East respiratory syndrome coronavirus, and, as of September 2021, SARS-CoV-2, confirmed using the Cepheid Xpert Xpress SARS-CoV2 RT-PCR kit.
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